Cold-active hydrolytic enzymes production by psychrotrophic Staphylococcus eqourum strain QCM1 isolated from refrigerated milk
Syed Nisar Ahmed, Syeda Kulsoom Fatema, Mohammad Shakir
Psychrophiles are the most abundant extremophiles in terms of biomass, diversity, and distribution. Cold-adapted microorganisms can be found in large areas of the planet, including the oceans 70 percent and the Polar Regions (20 percent). Man-made habitats (such as refrigeration and freezer systems) make up a small portion of the total number of potential habitats for cold-adapted organisms. In recent years, there has been a lot of research into the diversity of microorganisms that live in cold environments, with a focus on culture-dependent and culture-independent techniques. In the current investigation Staphylococcus eqourum was isolated from refrigerated milk by serial dilution method and plate count was taken. psychrotrophic count was between 60-91 ×102 CFU/ml. Among the bacterial isolates, Staphylococcus eqourum was obtained and were further confirmed by using Bergey’s manual of Determinative bacteriology (9th edition) and 16 S rRNA sequencing. Staphylococcus eqourum showed significant hydrolytic potential with different temperatures and pH. Optimum proteolytic activity of43 U/ml at 200C pH (7) was showed by bacterium. It goes on decreasing with increase in temperature and pH as well as at lower temperature and pH. Lipolytic activity of Staphylococcus eqourum strain QCM1was observed as 18.3 U/ml at 200C and 18.6 U/ml at pH (7). Our results suggest that Staphylococcus eqourum strain QCM1 is capable of producing hydrolytic cold active enzymes and can be studied further for investigation of their hydrolytic enzymes.
Syed Nisar Ahmed, Syeda Kulsoom Fatema, Mohammad Shakir. Cold-active hydrolytic enzymes production by psychrotrophic Staphylococcus eqourum strain QCM1 isolated from refrigerated milk. European Journal of Biotechnology and Bioscience, Volume 9, Issue 2, 2021, Pages 01-05