in vitro protocol optimization for mass propagation of pineapple (Ananas comosus L.) cv. smooth cayenne
Pineapple is the third most important tropical fruit in the world, after the banana and citrus. The fruits are important source of vitamin A, B and C and contain a protein digesting enzyme bromelain. Pineapple fruits are consumed fresh or processed into canned fruit, juice or jam. Micro propagation technique (Plant tissue culture) offers an opportunity for large scale production of uniform pineapple planting material in a relatively short period of time. The result showed that contamination, survival rate and brown of Mancozeb 80%WP at 0.6 g/L more clean, survival rate and less brown 8.4, 91.6 and 33.1% respectively, copper sulfate at 0.6 g/L less contamination, high survival rate and less brown 12.6 89.4 and 35.3% respectively and at 5% active chlorine had also effective to clean explants, less phenol and higher survival rate in the treatment combination of the experimental results at 30% (V/V) of NaOCl were 10.5, 49.8 and 92.5 % respectively. The highest shoot proliferation were recorded at 2.5 mg/l BA in combination with 0.5 mg/l KN 58.6 shoots/explant in full MS media and the highest root induction also recorded at 1 mg/l NAA 10.67 root /shootlet in half strength MS media. Pineapple was highly adapted for micro climate of greenhouse and the seedling were planted in the soil media of top soil, vermin compost and sand 0:1:2 ratios respectively have been acclimatized 99.6% in primary for a month. Thus, efficient protocol has been established for the mass propagation of the improved pineapple smooth cayenne cultivar.
Berihu Mengs. in vitro protocol optimization for mass propagation of pineapple (Ananas comosus L.) cv. smooth cayenne. European Journal of Biotechnology and Bioscience, Volume 9, Issue 2, 2021, Pages 28-33