Asymbiotic seed germination and micropropagation of Acampe rigida (Buch.-Ham. ex J. E. Sm.) P. F. Hunt through in vitro culture
Tapash Kumar Bhowmik, Mahbubur Rahman
Seeds of an endangered epiphytic orchid Acampe rigida were aseptically germinated on agar solidified MS, PM, MVW and KC media supplemented with sucrose, glucose and lactose separately. Higher germination was achieved on sucrose containing PM medium. Highest mean increased seedlings length was achieved on liquid PM with 0.5 mg/l NAA + 1.0 mg/l BAP followed by semisolid PM medium with 1.0 mg/l IAA + 1.0 mg/l BAP. The nodal segments directly produced the highest number of multiple shoot buds (9.02 ± 0.47/segment) on MS medium + 0.5 mg/l NAA + 1.0 mg/l BAP followed by 8.76 ± 0.62 shoot buds/segment produced on MS + 1.0 mg/l IAA + 2.0 mg/l BAP. Leaf segments underwent proliferation and produced maximum greenish PLBs (Protocorm Like Bodies) on MS medium supplemented with 1.0 mg/l Pic + 2.0 mg/l BAP followed by yellowish PLBs on MS + 1.0 mg/l IBA + 2.0 mg/l BAP. The highest mean increased individual shoot bud length was achieved on liquid PM (2.56 ± 0.20cm) with 1.0 mg/l IAA + 1.0 mg/l BAP followed by on agar solidified MS (2.53 ± 0.15cm) with 0.5 mg/l NAA + 1.0 mg/l BAP medium. The highest number of SPSs (Shoot Primordia Like Structures) formation took place in liquid MS + 0.5 mg/l Pic + 1.0 mg/l BAP. The increase in length as well as the number of roots developed seed originated and shoot bud derived seedlings were more on MS medium + 1.0 mg/l NAA followed by that on half strength MS0 medium + with 1.5% (w/v) sucrose containing rooting media. This efficient protocol of Acampe rigida could be used in orchid industry for the in vitro germination, SPSs production and micropropagation.
Tapash Kumar Bhowmik, Mahbubur Rahman. Asymbiotic seed germination and micropropagation of Acampe rigida (Buch.-Ham. ex J. E. Sm.) P. F. Hunt through in vitro culture. European Journal of Biotechnology and Bioscience, Volume 9, Issue 4, 2021, Pages 39-46